Publications

Selected Publications:

Tsatsaronis JA, Franch-Arroyo S, Resch U, Charpentier E. 2018. Extracellular Vesicle RNA: A Universal Mediator of Microbial Communication? Trends in Microbiology 26(5):401-410.
PubMed Link

Hille F, Richter H, Wong SP, Bratovič M, Ressel S, Charpentier E. 2018. The biology of CRISPR-Cas: Backward and forward. Cell 172(6):1239-1259.
PubMed Link

Charpentier E. 2018. Spotlight on… Emmanuelle Charpentier. FEMS Microbiol Lett. 365(4).
PubMed Link

Labuhn M, Adams FF, Ng M, Knoess S, Schambach A, Charpentier EM, Schwarzer A, Mateo JL, Klusmann JH, Heckl D. 2018. Refined sgRNA efficacy prediction improves large- and small-scale CRISPR-Cas9 applications. Nucleic Acids Res. 46(3):1375-1385.
PubMed Link

Hurwitz JL, Jones BG, Charpentier E, Woodland DL. 2018. Hypothesis: RNA and DNA Viral Sequence Integration into Mammalian Host Genome Supports Long-Term B Cell and T Cell Adaptive Immunity. Viral Immunol. 30(9):628-632.
PubMed Link

Fabiani FD, Renault TT, Peters B, Dietsche T, Gálvez EJC, Guse A, Freier K, Charpentier E, Strowig T, Franz-Wachtel M, Macek B, Wagner S, Hensel M and Erhardt M. 2017. A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum. PLoS Biol. 15(8):e2002267.
PubMed Link

Elsholz AKW, Birk MS, Charpentier E and Turgay K. 2017. Functional diversity of AAA+ protease complexes in Bacillus subtilis. Front. Mol. Biosci. 4:44.
PubMed Link

Reimer J, Knöss S, Labuhn M, Charpentier E, Göhring G, Schlegelberger B, Klusmann JH and Heckl D. 2017. CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo. Haematologica 102(9):1558-1566.
PubMed Link

Richter F, Fonfara I, Gelfert R, Nack J, Charpentier E and Möglich A. 2017. Switchable Cas9. Curr. Opin. Biotechnol. 48:119-126.
PubMed Link

Renault TT, Abraham AO, Bergmiller T, Paradis G, Rainville S, Charpentier E, Guet CC, Tu Y, Namba K, Keener JP, Minamino T and Erhardt M. 2017. Bacterial flagella grow through an injection-diffusion mechanism. Elife e23136.
PubMed Link

Le Rhun A, Lécrivain AL, Reimegård J, Proux-Wéra E, Broglia L, Della Beffa C and Charpentier E. 2017. Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III in Streptococcus pyogenes. Nucleic Acids Res. 45(5):2329-2340.
PubMed Link

Eggenschwiler R, Moslem M, Fráguas MS, Galla M, Papp O, Naujock M, Fonfara I, Gensch I, Wähner A, Beh-Pajooh A, Mussolino C, Tauscher M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, Cathomen T and Cantz T. 2016. Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci Rep. 6:38198.
PubMed Link

Resch U, Tsatsaronis JA, Le Rhun A, Stübiger G, Rohde M, Kasvandik S, Holzmeister S, Tinnefeld P, Wai SN and Charpentier E. 2016. A two-component regulatory system impacts extracellular membrane-derived vesicle production in group A streptococcus. MBio 7(6):e00207-e00216.
PubMed Link

Richter F, Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier E and Möglich A. 2016. Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Res. 44(20):10003-10014.
PubMed Link

Hille F and Charpentier E. 2016. CRISPR-Cas: biology, mechanisms and relevance. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 371(1707):20150496.
PubMed Link

Labenski V, Suerth JD, Barczak E, Heckl D, Levy C, Bernadin O, Charpentier E, Williams DA, Fehse B, Verhoeyen E and Schambach A. 2016. Alpharetroviral self-inactivating vectors produced by a superinfection-resistant stable packaging cell line allow genetic modification of primary human T lymphocytes. Biomaterials 97:97-109.
PubMed Link

Fonfara I, Richter H, Bratovič M, Le Rhun A and Charpentier E. 2016. The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA. Nature 532(7600):517-521.
PubMed Link

Sternberg SH, Richter H, Charpentier E and Qimron U. 2016. Adaptation in CRISPR-Cas systems. Mol. Cell 61(6):797-808.
PubMed Link

Le Rhun A, Beer YY, Reimegård J, Chylinski K and Charpentier E. 2016. RNA sequencing uncovers antisense RNAs and novel small RNAs in Streptococcus pyogenes. RNA Biol. 13(2):177-195.
PubMed Link

Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R and Koonin EV. 2015. An updated evolutionary classification of CRISPR-Cas systems. Nat. Rev. Microbiol. 13(11):722-736.
PubMed Link

Charpentier E and Hess WR. 2015. Editorial: RNA in bacteria: biogenesis, regulatory mechanisms and functions. FEMS Microbiol. Rev. 39(3):277-279.
PubMed Link

Heckl D and Charpentier E. 2015. Toward whole-transcriptome editing with CRISPR-Cas9. Mol. Cell 58(4):560-562.
PubMed Link

Charpentier E, Richter H, van der Oost J and White MF. 2015. Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity. FEMS Microbiol. Rev. 39(3):428-441.
PubMed Link

Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna JA, Feng G, Greely HT, Isasi R, Ji W, Kim JS, Knoppers B, Lanphier E, Li J, Lovell-Badge R, Martin GS2, Moreno J, Naldini L, Pera M, Perry AC, Venter JC, Zhang F and Zhou Q. 2015. CRISPR germline engineering-the community speaks. Nat. Biotechnol. 33(5):478-486.
PubMed Link

Plagens A, Richter H, Charpentier E and Randau L. 2015. DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes. FEMS Microbiol. Rev. 39(3):442-463.
PubMed Link

Charpentier E. 2015. CRISPR-Cas9: how research on a bacterial RNA-guided mechanism opened new perspectives in biotechnology and biomedicine. EMBO Mol. Med. 7(4):363-365.
PubMed Link

Doudna JA and Charpentier E. 2014. The new frontier of genome engineering with CRISPR-Cas9. Science 346(6213):1258096.
PubMed Link

Latvala S, Mäkelä SM, Miettinen M, Charpentier E and Julkunen I. 2014. Dynamin inhibition interferes with inflammasome activation and cytokine gene expression in Streptococcus pyogenes-infected human macrophages. 2014. Clin. Exp. Immunol. 178(2):320-333.
PubMed Link

Charpentier E and Marraffini LA. 2014. Harnessing CRISPR-Cas9 immunity for genetic engineering. Curr. Opin. Microbiol. 19:114-119.
PubMed Link

Charpentier E and Marraffini LA. 2014. Novel technologies in microbiology: Recent advances in techniques in microbiology. Curr. Opin. Microbiol. 19:viii-x.
PubMed Link

Chylinski K, Makarova KS, Charpentier E and Koonin EV. 2014. Classification and evolution of type II CRISPR-Cas systems. Nucleic Acids Res. 42(10):6091-6105.
PubMed Link

Jinek M, Jiang F, Taylor DW, Stenberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E and Doudna JA. 2014. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science 343(6176):1247997.
PubMed Link

Fonfara I, Le Rhun A, Chylinski K, Makarova KS, Lécrivain AL, Bzdrenga J, Koonin EV and Charpentier E. 2014. Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems. Nucleic Acids Res. 42(4):2577-2590.
PubMed Link

Fuhrmann J, Mierzwa B, Trentini DB, Spiess S, Lehner A, Charpentier E and Clausen T. 2013. Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in Gram-positive bacteria. Cell Rep. 3(6):1832-1839.
PubMed Link

Chylinski K, Le Rhun A and Charpentier E. 2013. The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems. RNA Biol. 10(5):726-737.
PubMed Link

Charpentier E and Doudna JA. 2013. Biotechnology: Rewriting a genome. Nature 495(7439):50-51.
PubMed Link

Fineran PC and Charpentier E. 2012. Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information. Virology 434(2):202-209.
PubMed Link

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA and Charpentier E. 2012. A programmable dual-RNA guided DNA endonuclease in adaptive bacterial immunity. Science 337(6096):816-821.
PubMed Link

Le Rhun A and Charpentier E. 2012. Small RNAs in streptococci. RNA Biol. 9(4):414-426.
PubMed Link

Makarova KS, Haft DH, Barrangou R, Brouns SJ, Charpentier E, Horvath P, Moineau S, Mojica FJ, Wolf YI, Yakunin A, van der Oost J and Koonin EV. 2011. Evolution and classification of the CRISPR/Cas systems. Nat. Rev. Microbiol. 9(6):467-477.
PubMed Link

Deltcheva E, Chylinski K, Sharma C, Gonzales K, Chao Y, Pirzada ZA, Eckert M, Vogel J and Charpentier E. 2011. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 471(7340):602-607.
PubMed Link