In addition to the CRISPR-associated RNAs, bacteria encode multiple other small RNAs (sRNAs) that play critical regulatory functions in major biological pathways. We have identified a number of putative cis-acting sRNAs (e.g. riboswitches) and trans-acting sRNAs in the human pathogen Streptococcus pyogenes. We are addressing the question of how selected sRNAs integrate into the general regulatory network controlling pathogenesis and related mechanisms in this pathogen. We want to understand the regulation of sRNA expression by ribonucleases, analyze the biological functions of sRNAs, identify their interacting partners and determine their modes of action at the molecular and cellular level.